2004 SIVB Congress Symposium Proceeding: Transgene Management via Multiple Site-specific Recombination Systems

Utilization of Site-Specific Recombination in Biopharmaceutical Production

Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and pr...

Site‐specific recombination

utilization of site-specific recombination in biopharmaceutical production

mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. several recombinant protein systems have been introduced to the market, most of which are under clinical development. in spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and pr...

Site-specific recombination of a transgene in fertilized eggs by transient expression of Cre recombinase.

An efficient method of transgene modulation in fertilized eggs has been developed that uses the Cre/loxP recombination system. Twelve transgenic mouse lines carrying a chicken beta-actin promoter-loxP-chloramphenicol acetyltransferase (CAT) gene-loxP-beta-galactosidase gene construct were produced. After selection of the line showing the highest expression of the CAT gene in a variety of tissue...

Microinjection of cre recombinase RNA induces site-specific recombination of a transgene in mouse oocytes.

We have developed a strategy for producing single copy transgenic mouse lines using Cre-loxP site specific recombination. The method is based on transient expression of the recombinase after injection of in vitro transcribed mRNA into the cytoplasm of fertilised eggs containing multiple copies of the transgene. The success rate of the recombination event is 100% (15 out of 15).